Gene description for NCL
Gene name	nucleolin
Gene symbol	NCL
Other names/aliases	C23 Nsr1
Species	Homo sapiens
Experiment description of studies that identified NCL in Melanoma cells
1 Experiment ID 617 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry [LTQ] PubMed ID 27894104    Organism Homo sapiens Experiment description Proteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers. Authors Hurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr. Journal name Oncotarget Publication year 2016 Sample Melanoma cells Sample name LOX IMVI Isolation/purification methods Differential centrifugation Polymer-based precipitation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] EV-TRACK -
2 Experiment ID 618 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry [LTQ] PubMed ID 27894104    Organism Homo sapiens Experiment description Proteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers. Authors Hurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr. Journal name Oncotarget Publication year 2016 Sample Melanoma cells Sample name M14 Isolation/purification methods Differential centrifugation Polymer-based precipitation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] EV-TRACK -
3 Experiment ID 619 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry [LTQ] PubMed ID 27894104    Organism Homo sapiens Experiment description Proteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers. Authors Hurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr. Journal name Oncotarget Publication year 2016 Sample Melanoma cells Sample name MALME-3M Isolation/purification methods Differential centrifugation Polymer-based precipitation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] EV-TRACK -
4 Experiment ID 620 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry [LTQ] PubMed ID 27894104    Organism Homo sapiens Experiment description Proteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers. Authors Hurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr. Journal name Oncotarget Publication year 2016 Sample Melanoma cells Sample name MDA-MB-435 Isolation/purification methods Differential centrifugation Polymer-based precipitation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] EV-TRACK -
5 Experiment ID 622 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry [LTQ] PubMed ID 27894104    Organism Homo sapiens Experiment description Proteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers. Authors Hurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr. Journal name Oncotarget Publication year 2016 Sample Melanoma cells Sample name SK-MEL-28 Isolation/purification methods Differential centrifugation Polymer-based precipitation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] EV-TRACK -
6 Experiment ID 623 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry [LTQ] PubMed ID 27894104    Organism Homo sapiens Experiment description Proteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers. Authors Hurwitz SN, Rider MA, Bundy JL, Liu X, Singh RK, Meckes DG Jr. Journal name Oncotarget Publication year 2016 Sample Melanoma cells Sample name SK-MEL-5 Isolation/purification methods Differential centrifugation Polymer-based precipitation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] EV-TRACK -
7 Experiment ID 986 Identified molecule protein Extracellular vesicle type Exosomes/Microvesicles MISEV EVs Identification method Mass spectrometry PubMed ID 25261472    Organism Homo sapiens Experiment description Complex N-linked glycans serve as a determinant for exosome/microvesicle cargo recruitment. Authors Liang Y, Eng WS, Colquhoun DR, Dinglasan RR, Graham DR, Mahal LK. Journal name J Biol Chem. Publication year 2014 Sample Melanoma cells Sample name SK-MEL-5 Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry Western blotting EV-TRACK EV140188: EV-METRIC:22%
8 Experiment ID 1624 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Western blotting PubMed ID 34012515    Organism Homo sapiens Experiment description CD9 inhibition reveals a functional connection of extracellular vesicle secretion with mitophagy in melanoma cells Authors Suárez H, Andreu Z, Mazzeo C, Toribio V, Pérez-Rivera AE, López-Martín S, García-Silva S, Hurtado B, Morato E, Peláez L, Arribas EA, Tolentino-Cortez T, Barreda-Gómez G, Marina AI, Peinado H, Yáñez-Mó M. Journal name J Extracell Vesicles Publication year 2021 Sample Melanoma cells Sample name SK‐MEL‐147 Isolation/purification methods Differential centrifugation Centrifugal ultrafiltration Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study LC-MS/MS Western blotting EV-TRACK -
9 Experiment ID 1625 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Western blotting PubMed ID 34012515    Organism Homo sapiens Experiment description CD9 inhibition reveals a functional connection of extracellular vesicle secretion with mitophagy in melanoma cells Authors Suárez H, Andreu Z, Mazzeo C, Toribio V, Pérez-Rivera AE, López-Martín S, García-Silva S, Hurtado B, Morato E, Peláez L, Arribas EA, Tolentino-Cortez T, Barreda-Gómez G, Marina AI, Peinado H, Yáñez-Mó M. Journal name J Extracell Vesicles Publication year 2021 Sample Melanoma cells Sample name SK‐MEL‐147 Isolation/purification methods Differential centrifugation Centrifugal ultrafiltration Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study LC-MS/MS Western blotting EV-TRACK -
10 Experiment ID 1626 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Western blotting PubMed ID 34012515    Organism Homo sapiens Experiment description CD9 inhibition reveals a functional connection of extracellular vesicle secretion with mitophagy in melanoma cells Authors Suárez H, Andreu Z, Mazzeo C, Toribio V, Pérez-Rivera AE, López-Martín S, García-Silva S, Hurtado B, Morato E, Peláez L, Arribas EA, Tolentino-Cortez T, Barreda-Gómez G, Marina AI, Peinado H, Yáñez-Mó M. Journal name J Extracell Vesicles Publication year 2021 Sample Melanoma cells Sample name SK‐MEL‐147 Isolation/purification methods Differential centrifugation Centrifugal ultrafiltration Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study LC-MS/MS Western blotting EV-TRACK -
11 Experiment ID 1627 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Western blotting PubMed ID 34012515    Organism Homo sapiens Experiment description CD9 inhibition reveals a functional connection of extracellular vesicle secretion with mitophagy in melanoma cells Authors Suárez H, Andreu Z, Mazzeo C, Toribio V, Pérez-Rivera AE, López-Martín S, García-Silva S, Hurtado B, Morato E, Peláez L, Arribas EA, Tolentino-Cortez T, Barreda-Gómez G, Marina AI, Peinado H, Yáñez-Mó M. Journal name J Extracell Vesicles Publication year 2021 Sample Melanoma cells Sample name SK‐MEL‐147 Isolation/purification methods Differential centrifugation Centrifugal ultrafiltration Size exclusion chromatography Flotation density - Molecules identified in the study Protein Lipid Methods used in the study LC-MS/MS MALDI-MS Western blotting EV-TRACK -
12 Experiment ID 1628 Identified molecule protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Western blotting PubMed ID 34012515    Organism Homo sapiens Experiment description CD9 inhibition reveals a functional connection of extracellular vesicle secretion with mitophagy in melanoma cells Authors Suárez H, Andreu Z, Mazzeo C, Toribio V, Pérez-Rivera AE, López-Martín S, García-Silva S, Hurtado B, Morato E, Peláez L, Arribas EA, Tolentino-Cortez T, Barreda-Gómez G, Marina AI, Peinado H, Yáñez-Mó M. Journal name J Extracell Vesicles Publication year 2021 Sample Melanoma cells Sample name SK‐MEL‐147 Isolation/purification methods Differential centrifugation Centrifugal ultrafiltration Size exclusion chromatography Flotation density - Molecules identified in the study Protein Lipid Methods used in the study LC-MS/MS MALDI-MS Western blotting EV-TRACK -
13 Experiment ID 1624 Identified molecule Protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry PubMed ID 34012515    Organism Homo sapiens Experiment description CD9 inhibition reveals a functional connection of extracellular vesicle secretion with mitophagy in melanoma cells Authors Suárez H, Andreu Z, Mazzeo C, Toribio V, Pérez-Rivera AE, López-Martín S, García-Silva S, Hurtado B, Morato E, Peláez L, Arribas EA, Tolentino-Cortez T, Barreda-Gómez G, Marina AI, Peinado H, Yáñez-Mó M. Journal name J Extracell Vesicles Publication year 2021 Sample Melanoma cells Sample name SK‐MEL‐147 Isolation/purification methods Differential centrifugation Centrifugal ultrafiltration Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study LC-MS/MS Western blotting EV-TRACK -
14 Experiment ID 1625 Identified molecule Protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry PubMed ID 34012515    Organism Homo sapiens Experiment description CD9 inhibition reveals a functional connection of extracellular vesicle secretion with mitophagy in melanoma cells Authors Suárez H, Andreu Z, Mazzeo C, Toribio V, Pérez-Rivera AE, López-Martín S, García-Silva S, Hurtado B, Morato E, Peláez L, Arribas EA, Tolentino-Cortez T, Barreda-Gómez G, Marina AI, Peinado H, Yáñez-Mó M. Journal name J Extracell Vesicles Publication year 2021 Sample Melanoma cells Sample name SK‐MEL‐147 Isolation/purification methods Differential centrifugation Centrifugal ultrafiltration Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study LC-MS/MS Western blotting EV-TRACK -
15 Experiment ID 1626 Identified molecule Protein Extracellular vesicle type Extracellular vesicles MISEV EVs Identification method Mass spectrometry PubMed ID 34012515    Organism Homo sapiens Experiment description CD9 inhibition reveals a functional connection of extracellular vesicle secretion with mitophagy in melanoma cells Authors Suárez H, Andreu Z, Mazzeo C, Toribio V, Pérez-Rivera AE, López-Martín S, García-Silva S, Hurtado B, Morato E, Peláez L, Arribas EA, Tolentino-Cortez T, Barreda-Gómez G, Marina AI, Peinado H, Yáñez-Mó M. Journal name J Extracell Vesicles Publication year 2021 Sample Melanoma cells Sample name SK‐MEL‐147 Isolation/purification methods Differential centrifugation Centrifugal ultrafiltration Size exclusion chromatography Flotation density - Molecules identified in the study Protein Methods used in the study LC-MS/MS Western blotting EV-TRACK -